Altered fractionation profiles of higher plant poly(A)-containing RNA by selective formation of a complex with a hydrophobic impurity

Hideki Takahashi, Takeshi Nitta

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Synthetic polyadenylate (poly(A)) which originally sedimented at 6-7 S in a sucrose density gradient did so at 15-19 S in the presence of phenol-extracted nucleic acids from higher plant tissues. After centrifugation, the input poly(A) was found to be insensitive to RNase T2 (EC 3.129.1), suggesting a newly-formed complex with a hydrophobic substance contaminating the nucleic acid preparation. The artifically-formed poly(A)-impurity complex migrated more slowly than unbound poly(A) during gel electrophoresis. The complex was retained rather effectively by an oligodeoxythymidylate (oligo-(dT))-cellulose column and a polyuridylate (poly(U))-glassfiber filter. However, a nitrocellulose membrane filter retained the poly(A)-complex to various extents depending on plant materials, suggesting different varieties of the poly(A)-binding impurity in the higher plant kingdom. Fractionation of mRNA by these ordinarily used procedures is suggested to be variously altered by complex formation with this hydrophobic impurity.

本文言語English
ページ(範囲)63-67
ページ数5
ジャーナルPlant Science
43
1
DOI
出版ステータスPublished - 1986 1 1
外部発表はい

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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