Allelotype analysis of gallbladder carcinoma associated with anomalous junction of pancreaticobiliary duct

Kentaro Nakayama, Miki Konno, Atsuko Kanzaki, Takanori Morikawa, Hitoshi Miyashita, Toru Fujioka, Takafumi Uchida, Kohji Miyazaki, Sonshin Takao, Takashi Aikou, Manabu Fukumoto, Yuji Takebayashi

研究成果: Article

24 被引用数 (Scopus)

抄録

Anomalous junction of pancreaticobiliary duct (AJPBD) patients has an increased risk of gallbladder and bile duct carcinomas. However, the relevance of carcinoma with AJPBD is not fully clarified. We performed analysis of loss of heterozygosity (LOH) at p53 locus and immunohistochemistry of p53 and K-ras gene mutation in five cases of gallbladder carcinoma associated with AJPBD. LOH of p53 locus and overexpression of p53 were detected in two out of five (40%) and five out of five (100%), respectively, in the present study. K-ras gene mutation at codon 12 and 13 was not detected (0%, 0/5). These results suggest that aberrations of p53 are involved in carcinogenesis of gallbladder carcinoma associated with AJPBD. Next, in order to find the genetic events besides K-ras mutation and overexpression of mutant p53 in this disease, LOH analysis was performed using 72 microsatellite markers. High frequency of allelic loss (>50%) was found on 2p (81.8%), 4p (50%), 4q (50%), 8q (60%), 9q (50%), 10p (50%), 14p (60%), 14q (50%), 16p (60%), 19p (50%), 21p (50%) and Xp (66.6%). The highest deletion regions on chromosome 2p24 (3/3, 100%), 14q22 (3/4, 75%) and 21q22 (3/4, 75%) were found. The present study suggests that gallbladder carcinoma associated with AJPBD has high frequent allelic loss and has two new regions which may harbor putative tumor suppressor genes.

本文言語English
ページ(範囲)135-141
ページ数7
ジャーナルCancer Letters
166
2
DOI
出版ステータスPublished - 2001 5 26

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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