The adsorption behaviors of R-propranolol (PPL) for the negatively-charged liposomes were studied focusing on the liposome membrane properties. In the case of the negatively-charged DMPA and DMPS liposomes, the adsorbed amount of PPL on the membrane surface was high, while little amount of PPL did on the neutral DMPC liposome. Using fluorescent probes, the membrane properties such as membrane fluidity and membrane polarity were investigated. The PPL adsorption on the negatively-charged liposomes induced an increase in membrane fluidity, and the membrane surface turned to be hydrated. Raman spectroscopic analysis revealed the decrease of packing density of DMPA and DMPS liposomes in the presence of PPL molecules. It is considered that the PPL molecules can adsorb on the membranes through not only electrostatic interaction but also other weak interactions such as hydrophobic interaction and hydrogen bond interaction. These findings also suggest the significance of the membrane properties, i.e., surface charge, membrane fluidity, and membrane polarity, in understanding the adsorption behaviors of chemicals on the self-assembled membranes.
ASJC Scopus subject areas
- 化学 (全般)