ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects

Yudai Tokumasu, Atsuo Iida, Zi Wang, Satoshi Ansai, Masato Kinoshita, Atsuko Sehara-Fujisawa

研究成果: Article査読

8 被引用数 (Scopus)

抄録

ADAM (a disintegrin and metalloprotease) constitutes a family of multi-domain proteins that are involved in development, homeostasis, and disease. ADAM12 plays important roles in myogenesis and adipogenesis in mice; however, the precise physiological mechanisms are not known, and the function of this gene in other vertebrates has not been examined. In this study, we used a simple model vertebrate, the zebrafish, to investigate the functions of ADAM12 during development. Zebrafish adam12 is conserved with those of mammals in the synteny and the amino-acid sequence. We examined adam12 expression in zebrafish embryos by whole mount in situ hybridization and the promoter activity of the adam12 upstream sequence. We found that adam12 is strongly expressed in the cardiovascular system, erythroid progenitors, brain, and jaw cartilage during zebrafish development, and adam12-knockout zebrafish exhibited reduced body size in the juvenile stage without apparent morphological defects. Taken together, these results suggest that adam12 plays a significant role in the regulation of body growth during juvenile stage in zebrafish, although the precise molecular mechanisms await further study.

本文言語English
ページ(範囲)409-421
ページ数13
ジャーナルDevelopment Growth and Differentiation
58
4
DOI
出版ステータスPublished - 2016 5月 1
外部発表はい

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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