TY - JOUR
T1 - Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010
AU - Ikeda, Tatsuya
AU - Mizuta, Katsumi
AU - Abiko, Chieko
AU - Aoki, Yoko
AU - Itagaki, Tsutomu
AU - Katsushima, Fumio
AU - Katsushima, Yuriko
AU - Matsuzaki, Yoko
AU - Fuji, Naoko
AU - Imamura, Tadatsugu
AU - Oshitani, Hitoshi
AU - Noda, Masahiro
AU - Kimura, Hirokazu
AU - Ahiko, Tadayuki
PY - 2012/2
Y1 - 2012/2
N2 - To clarify the epidemiology of enterovirus 68 (EV68), which is one of the most rarely identified enteroviruses, virus isolation and molecular screening using RT-PCR was performed on 6307 respiratory specimens collected at pediatric clinics in Yamagata, Japan between 2005 and 2010. In the years 2005-2009, 10, 1, 2, 0, and 2 (40) EV68-positive cases, respectively, were identified by RT-PCR. In 2010, 40 cases were identified altogether: 2 by isolation only, 26 by RT-PCR only, and 12 by both isolation and RT-PCR. Phylogenetic analysis indicated that plural genetically distinct clusters co-circulated. These results suggest that that difficulty in EV68 isolation leads to an underestimation of the prevalence of EV68 infections.
AB - To clarify the epidemiology of enterovirus 68 (EV68), which is one of the most rarely identified enteroviruses, virus isolation and molecular screening using RT-PCR was performed on 6307 respiratory specimens collected at pediatric clinics in Yamagata, Japan between 2005 and 2010. In the years 2005-2009, 10, 1, 2, 0, and 2 (40) EV68-positive cases, respectively, were identified by RT-PCR. In 2010, 40 cases were identified altogether: 2 by isolation only, 26 by RT-PCR only, and 12 by both isolation and RT-PCR. Phylogenetic analysis indicated that plural genetically distinct clusters co-circulated. These results suggest that that difficulty in EV68 isolation leads to an underestimation of the prevalence of EV68 infections.
KW - Acute respiratory infection
KW - Enterovirus 68
KW - Reverse transcription polymerase chain reaction
KW - Virus isolation
UR - http://www.scopus.com/inward/record.url?scp=84858202985&partnerID=8YFLogxK
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U2 - 10.1111/j.1348-0421.2012.00411.x
DO - 10.1111/j.1348-0421.2012.00411.x
M3 - Comment/debate
C2 - 22309616
AN - SCOPUS:84858202985
VL - 56
SP - 139
EP - 143
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 2
ER -