Acute changes in the axonal cystoskeleton after mild stretching of the rat brachial plexus

Kenshi Kikukawa, Kohji Fukunaga, Teiji Kato, Makio Yamaga, Eishichi Miyamoto, Katsumasa Takagi

研究成果: Article査読

2 被引用数 (Scopus)

抄録

We have developed an animal model to investigate acute changes in the axonal cytoskeleton caused by a mild stretching of the peripheral nerve in the upper limbs of rats. Rat forelimbs were continuously stretched at 2 N for 1 h. Thereafter, a part of the brachial plexus and median nerve were harvested and processed for electron microscopic analysis. The total number of microtubules in the brachial plexus decreased to 55% of that of the control animals (p < 0.05) without change in the number of neurofilaments. No significant changes in microtubules or neurofilaments were observed in the median nerve. By Western blotting analysis, the amount of tau protein in the stretch group significantly decreased in the brachial plexus but not in the median nerve. However, no significant changes in the amount of tubulin protein were observed in either the brachial plexus or median nerve. These results suggest that the microtubules were depolymerized by stretching of the brachial plexus and that the depolymerization may have been mediated by the decrease in the tau protein.

本文言語English
ページ(範囲)359-364
ページ数6
ジャーナルJournal of Orthopaedic Research
21
2
DOI
出版ステータスPublished - 2003
外部発表はい

ASJC Scopus subject areas

  • 整形外科およびスポーツ医学

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