Activity and expression of aspartate aminotransferase during the reproductive cycle of a fresh water fish, Clarias batrachus

A. S. Srivastava, I. Oohara, T. Suzuki, S. N. Singh

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Aspartate amino transferase (AspT; EC: 2.6.1.1) is one of the key enzyme of aspartate malate shuttle and non-essential amino acid metabolism. In order to clarify the change of the activities of both cytosolic (c-) and mitochondrial (m-) AspT isoenzymes during the different reproductive stages of Clarias batrachus, an air-breathing carnivorous freshwater fish, the c- and m- AspT activities were measured spectrophotometrically in liver, heart, kidney, brain and ovary during the pre-spawning, spawning and post-spawning phases of the annual reproductive cycle of this fish. Maximum activities were observed during the spawning phase, followed by pre- and post-spawning phases. The native agarose gel electrophoretic pattern revealed two AspT forms, c-AspT and m-AspT, throughout the pre- to post-spawning phases. However, the spawning phase was marked by the expression of an additional anionic form of c-AspT in all tissues studied. These results indicate a distinct utilization of aspartate particularly in the spawning phase in fish in the central metabolic path via transamination reactions, and suggest a control of the enzyme activity by the expression of the additional c-AspT gene, especially at the spawning phase.

本文言語English
ページ(範囲)243-250
ページ数8
ジャーナルFish Physiology and Biochemistry
20
3
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 水圏科学

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