TY - JOUR
T1 - Activity and expression of aspartate aminotransferase during the reproductive cycle of a fresh water fish, Clarias batrachus
AU - Srivastava, A. S.
AU - Oohara, I.
AU - Suzuki, T.
AU - Singh, S. N.
N1 - Funding Information:
The first author is grateful to The Science and Technology Agency, Japan and The University Grant Commission, India for providing the funds for this work.
PY - 1999
Y1 - 1999
N2 - Aspartate amino transferase (AspT; EC: 2.6.1.1) is one of the key enzyme of aspartate malate shuttle and non-essential amino acid metabolism. In order to clarify the change of the activities of both cytosolic (c-) and mitochondrial (m-) AspT isoenzymes during the different reproductive stages of Clarias batrachus, an air-breathing carnivorous freshwater fish, the c- and m- AspT activities were measured spectrophotometrically in liver, heart, kidney, brain and ovary during the pre-spawning, spawning and post-spawning phases of the annual reproductive cycle of this fish. Maximum activities were observed during the spawning phase, followed by pre- and post-spawning phases. The native agarose gel electrophoretic pattern revealed two AspT forms, c-AspT and m-AspT, throughout the pre- to post-spawning phases. However, the spawning phase was marked by the expression of an additional anionic form of c-AspT in all tissues studied. These results indicate a distinct utilization of aspartate particularly in the spawning phase in fish in the central metabolic path via transamination reactions, and suggest a control of the enzyme activity by the expression of the additional c-AspT gene, especially at the spawning phase.
AB - Aspartate amino transferase (AspT; EC: 2.6.1.1) is one of the key enzyme of aspartate malate shuttle and non-essential amino acid metabolism. In order to clarify the change of the activities of both cytosolic (c-) and mitochondrial (m-) AspT isoenzymes during the different reproductive stages of Clarias batrachus, an air-breathing carnivorous freshwater fish, the c- and m- AspT activities were measured spectrophotometrically in liver, heart, kidney, brain and ovary during the pre-spawning, spawning and post-spawning phases of the annual reproductive cycle of this fish. Maximum activities were observed during the spawning phase, followed by pre- and post-spawning phases. The native agarose gel electrophoretic pattern revealed two AspT forms, c-AspT and m-AspT, throughout the pre- to post-spawning phases. However, the spawning phase was marked by the expression of an additional anionic form of c-AspT in all tissues studied. These results indicate a distinct utilization of aspartate particularly in the spawning phase in fish in the central metabolic path via transamination reactions, and suggest a control of the enzyme activity by the expression of the additional c-AspT gene, especially at the spawning phase.
KW - AAT
KW - AspT
KW - GOT
KW - Isoenzymes
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U2 - 10.1023/A:1007783213963
DO - 10.1023/A:1007783213963
M3 - Article
AN - SCOPUS:0342515997
VL - 20
SP - 243
EP - 250
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
SN - 0920-1742
IS - 3
ER -