Activation of the p21CIP1/WAF1 promoter by bone morphogenetic protein-2 in mouse B lineage cells

Kenji Yamato, Shinichi Hashimoto, Takeshi Imamura, Hideo Uchida, Nobuo Okahashi, Takeyoshi Koseki, Akira Ishisaki, Masahiro Kizaki, Kohei Miyazono, Yasuo Ikeda, Tatsuji Nishihara

研究成果: Article査読

19 被引用数 (Scopus)

抄録

BMPs exert a negative growth effect on various types of cells. We have previously reported that BMP-2 inhibited the growth of HS-72 mouse hybridoma cells by inducing p21CIP1/WAF1 expression. In the present study, we demonstrated that BMP-2 activated the mouse p21CIP1/WAF1 promoter in HS-72 cells, and that a 29-base pair (b) region of the promoter (-1928/-1900 relative to the TATA box), conserved between mice and humans, was responsive to BMP-2 as well as expression of Smad1, Smad4, and constitutively active mutants of BMP type I receptors. Furthermore, an oligonucleotide containing the 29-b region was found to be associated with Smad4 and phosphorylated Smad1 in the nuclear extract of BMP-2-stimulated HS-72 cells. These results suggested that BMP-2 might activate p21CIP1/WAF1 transcription by inducing a binding of Smad4 and Smad1 to the 29-b region in HS-72 cells.

本文言語English
ページ(範囲)4383-4392
ページ数10
ジャーナルOncogene
20
32
DOI
出版ステータスPublished - 2001 7 19
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学
  • 癌研究

フィンガープリント

「Activation of the p21<sup>CIP1/WAF1</sup> promoter by bone morphogenetic protein-2 in mouse B lineage cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル