Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.
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