Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

Tomonobu Kusano, Kazuo Izaki, Hajime Takahashi

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.

本文言語English
ページ(範囲)1779-1785
ページ数7
ジャーナルAgricultural and Biological Chemistry
44
8
DOI
出版ステータスPublished - 1980 8月
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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