Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

Tomonobu Kusano, Kazuo Izaki, Hajime Takahashi

    研究成果: Article査読

    3 被引用数 (Scopus)

    抄録

    Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.

    本文言語English
    ページ(範囲)1779-1785
    ページ数7
    ジャーナルAgricultural and Biological Chemistry
    44
    8
    DOI
    出版ステータスPublished - 1980 8

    ASJC Scopus subject areas

    • 生化学、遺伝学、分子生物学(全般)
    • 農業および生物科学(全般)

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