TY - JOUR
T1 - Acrolein, an α,β-unsaturated carbonyl, inhibits both growth and PSII activity in the cyanobacterium synechocystis sp. PCC 6803
AU - Shimakawa, Ginga
AU - Iwamoto, Tatsuya
AU - Mabuchi, Tomohito
AU - Saito, Ryota
AU - Yamamoto, Hiroshi
AU - Amako, Katsumi
AU - Sugimoto, Toshio
AU - Makino, Amane
AU - Miyake, Chikahiro
PY - 2013/9/16
Y1 - 2013/9/16
N2 - In this study, we sought to determine whether and how an α,β-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200μM acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO2- dependent O2 evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200μM acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803.
AB - In this study, we sought to determine whether and how an α,β-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200μM acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO2- dependent O2 evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200μM acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803.
KW - Acrolein
KW - NADPH-dependent acrolein reduction (NAR)
KW - Photosynthesis
KW - Plant diabetes
KW - Reactive carbonyls (RCs)
UR - http://www.scopus.com/inward/record.url?scp=84883700359&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883700359&partnerID=8YFLogxK
U2 - 10.1271/bbb.130186
DO - 10.1271/bbb.130186
M3 - Article
C2 - 23924728
AN - SCOPUS:84883700359
VL - 77
SP - 1655
EP - 1660
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 8
ER -