Acrolein, an α,β-unsaturated carbonyl, inhibits both growth and PSII activity in the cyanobacterium synechocystis sp. PCC 6803

Ginga Shimakawa, Tatsuya Iwamoto, Tomohito Mabuchi, Ryota Saito, Hiroshi Yamamoto, Katsumi Amako, Toshio Sugimoto, Amane Makino, Chikahiro Miyake

研究成果: Article査読

13 被引用数 (Scopus)

抄録

In this study, we sought to determine whether and how an α,β-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200μM acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO2- dependent O2 evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200μM acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803.

本文言語English
ページ(範囲)1655-1660
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
77
8
DOI
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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