Acid hydrolysis of protein in a microcapillary tube for the recovery of tryptophan

Abayomi Peter Adebiyi, Dong Hao Jin, Tomohisa Ogawa, Koji Muramoto

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The acid hydrolysis of proteins was miniaturized and simplified by employing microcapillary tubes (100 μl in volume) with 6 M HCl containing 1% 2-mercaptoethanol and 3% phenol for an amino acid compositional analysis. The method not only eliminated the laborious evacuation step for the hydrolysis tube but also decreased the destruction of tryptophan during hydrolysis. The recovery of tryptophan was 79% by acid hydrolysis at 145°C for 4 h. Since the acid mixture could be removed under vacuum, the hydrolysate was subjected to an amino acid analysis without neutralization or dilution.

本文言語English
ページ(範囲)255-257
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
69
1
DOI
出版ステータスPublished - 2005 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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