TY - JOUR
T1 - Accelerated glucose metabolism in hyphae-dispersed Aspergillus oryzae is suitable for biological production
AU - Sakuragawa, Taku
AU - Wakai, Satoshi
AU - Zhang, Silai
AU - Kawaguchi, Hideo
AU - Ogino, Chiaki
AU - Kondo, Akihiko
N1 - Funding Information:
We gratefully acknowledge Professor Keietsu Abe (Tohoku University) for providing the A. oryzae AG-GAGΔ strain. This work was supported by Special Coordination Funds for Promoting Science and Technology, and by the Creation of Innovation Centers for Advanced Interdisciplinary Research Areas program (Innovative Bioproduction Kobe) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan. The authors declare no conflict of interest.
Funding Information:
We gratefully acknowledge Professor Keietsu Abe (Tohoku University) for providing the A. oryzae AG-GAGΔ strain. This work was supported by Special Coordination Funds for Promoting Science and Technology , and by the Creation of Innovation Centers for Advanced Interdisciplinary Research Areas program (Innovative Bioproduction Kobe) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan. The authors declare no conflict of interest.
Publisher Copyright:
© 2021 The Society for Biotechnology, Japan
PY - 2021/8
Y1 - 2021/8
N2 - Recently, a hyphae-dispersed type of filamentous fungus Aspergillus oryzae was constructed via genetic engineering, and industrial applications are expected due to the ease of handling and to the level of protein production properties. In this study, we constructed cellulase-expressing strains using wild-type and hyphae-dispersed strains to investigate the correlation between protein productivity and metabolism. Compared with the original strain, the hyphae-dispersed cellulase-expressing strain showed elevated cellulase activity, rapid glucose consumption, increased mycelial dry weight, an increased expression of cellulase genes, and activated respiration activity. Comparative metabolomic analysis showed fewer metabolites in the glycolysis and TCA cycles in the dispersed strains than in the original strains. These results indicate that the flux of carbohydrate metabolism in the hyphae-dispersed strains is smoother than that in the original strains. Such efficient metabolic flux would contribute to efficient energy conversion and to sufficient energy supply to anabolisms, such as mycelial growth and protein production. Our findings suggest that the hyphae-dispersed strains could be a useful host not only for protein production but also for the biological production of various chemicals such as organic acids.
AB - Recently, a hyphae-dispersed type of filamentous fungus Aspergillus oryzae was constructed via genetic engineering, and industrial applications are expected due to the ease of handling and to the level of protein production properties. In this study, we constructed cellulase-expressing strains using wild-type and hyphae-dispersed strains to investigate the correlation between protein productivity and metabolism. Compared with the original strain, the hyphae-dispersed cellulase-expressing strain showed elevated cellulase activity, rapid glucose consumption, increased mycelial dry weight, an increased expression of cellulase genes, and activated respiration activity. Comparative metabolomic analysis showed fewer metabolites in the glycolysis and TCA cycles in the dispersed strains than in the original strains. These results indicate that the flux of carbohydrate metabolism in the hyphae-dispersed strains is smoother than that in the original strains. Such efficient metabolic flux would contribute to efficient energy conversion and to sufficient energy supply to anabolisms, such as mycelial growth and protein production. Our findings suggest that the hyphae-dispersed strains could be a useful host not only for protein production but also for the biological production of various chemicals such as organic acids.
KW - Aspergillus oryzae
KW - Carbon metabolism
KW - Enzyme production
KW - Hyphae-dispersed strain
KW - Metabolomic analysis
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U2 - 10.1016/j.jbiosc.2021.03.017
DO - 10.1016/j.jbiosc.2021.03.017
M3 - Article
C2 - 33896702
AN - SCOPUS:85106591948
VL - 132
SP - 140
EP - 147
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 2
ER -