A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization

Yuen Jun Chung, Jos Jonkers, Hannah Kitson, Heike Fiegler, Sean Humphray, Carol Scott, Sarah Hunt, Yuejin Yu, Ichiko Nishijima, Arno Velds, Henne Holstege, Nigel Carter, Allan Bradley

研究成果: Article査読

56 被引用数 (Scopus)

抄録

Microarray-based comparative genomic hybridization (CGH) has become a powerful method for the genome-wide detection of chromosomal imbalances. Although BAC microarrays have been used for mouse CGH studies, the resolving power of these analyses was limited because high-density whole-genome mouse BAC microarrays were not available. We therefore developed a mouse BAC microarray containing 2803 unique BAC clones from mouse genomic libraries at 1-Mb intervals. For the general amplification of BAC clone DNA prior to spotting, we designed a set of three novel degenerate oligonucleotide-primed (DOP) PCR primers that preferentially amplify mouse genomic sequences while minimizing unwanted amplification of contaminating Escherichia coli DNA. The resulting 3K mouse BAC microarrays reproducibly identified DNA copy number alterations in cell lines and primary tumors, such as single-copy deletions, regional amplifications, and aneuploidy.

本文言語English
ページ(範囲)188-196
ページ数9
ジャーナルGenome Research
14
1
DOI
出版ステータスPublished - 2004 1
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 遺伝学(臨床)

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