A subtractive gene expression screen suggests a role for vanin-1 in testis development in mice

Yoshihiko Uehara, Chisato Mori, Tetsuo Noda, Kohei Shiota, Naomi Kitamura

研究成果: Article査読

56 被引用数 (Scopus)

抄録

The molecular pathways leading from indifferent mammalian gonad to either testis or ovary are not well understood. A number of genes, including the Y-linked sex determining gene SRY, have been shown to play roles in sex determination or differentiation, but there are clearly many missing elements to be found. We used suppression-subtractive hybridization to construct normalized cDNA libraries enriched for male-specific or female-specific transcripts in mouse fetal gonads. We describe the strategy used to efficiently screen these libraries for candidate sex-determination and gonado-genesis genes. One gene arising from these screens is vanin-1, which encodes a protein implicated in the induction of cell migration into the thymus. We find that vanin-1 is expressed male-specifically in Sertoli cells of the developing testis and may be involved in inducing cell migration from the adjacent mesonephros, a process known to be critical for testis development. This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems. (C) 2000 Wiley-Liss, Inc.

本文言語English
ページ(範囲)124-135
ページ数12
ジャーナルGenesis
27
3
出版ステータスPublished - 2000 9 14

ASJC Scopus subject areas

  • 遺伝学
  • 内分泌学
  • 細胞生物学

フィンガープリント

「A subtractive gene expression screen suggests a role for vanin-1 in testis development in mice」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル