A stabilization technique of wobbly images taken by the inclined centrifuge microscope

A. Shirai, T. Hayase

研究成果: Conference contribution

抄録

The authors have observed behavior of HL60 cells on endothelial cells cultured on a flat glass plate for the understanding of fundamental mechanism of the immune response of neutrophils using the inclined centrifuge microscope. The inclined centrifuge microscope applies a centrifugal force to the HL60 cells using a centrifuge and records motion of the cells on the plate as successive stroboscopic images synchronized with rotation of the rotor of the centrifuge. The successive images were wobbly by flatter of the rotor or vibration of the motor of the centrifuge. To stabilize the images, they drew cross lines on the glass plate, and adjusted the images by fixing the position of the crossing point of the lines. However, it was difficult to extract the lines accurately, because the lines were not clear on the low-contrast images. Therefore, in this research, the authors introduced the Hough transformation to extract the lines. Moreover, adherent cells on the plate were also used to compensate remaining shaking of the images. Consequently, the displacements of the images were decreased to almost within±1 pixel.

本文言語English
ホスト出版物のタイトル6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
ページ1570-1573
ページ数4
DOI
出版ステータスPublished - 2010 10 22
イベント6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore
継続期間: 2010 8 12010 8 6

出版物シリーズ

名前IFMBE Proceedings
31 IFMBE
ISSN(印刷版)1680-0737

Other

Other6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
CountrySingapore
CitySingapore
Period10/8/110/8/6

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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