A SMAP Gene Family Encoding ARF GTPase-Activating Proteins and Its Implication in Membrane Trafficking

Kenji Tanabe, Shunsuke Kon, Nobuyuki Ichijo, Tomo Funaki, Waka Natsume, Toshio Watanabe, Masanobu Satake

研究成果: Chapter

5 被引用数 (Scopus)

抄録

SMAP1 and SMAP2 proteins constitute a subfamily of the Arf-specific GTPase-activating proteins. Both SMAP proteins bind to clathrin heavy chains and are involved in the trafficking of clathrin-coated vesicles. In cells, SMAP1 regulates Arf6-dependent endocytosis of transferrin receptors from the coated pits of the plasma membrane, whereas SMAP2 regulates Arf1-dependent retrograde transport of TGN38 from the early endosome to the trans-Golgi network. The common and distinct features of SMAP1 and SMAP2 activity provide a valuable opportunity to examine the differential regulation of membrane trafficking by these two proteins. In this chapter, we describe several basic experimental procedures that have been used to study the regulation of membrane trafficking using SMAP proteins, including a GAP assay as well as procedures to study the transport of transferrin receptors and TGN38. In addition, a yeast two-hybrid system is described because of its utility in identifying novel molecules that interact with SMAP.

本文言語English
ホスト出版物のタイトルSmall GTPases in Disease, Part A
編集者William Balch, Alan Hall, Channing Der
ページ155-170
ページ数16
DOI
出版ステータスPublished - 2008

出版物シリーズ

名前Methods in Enzymology
438
ISSN(印刷版)0076-6879

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

フィンガープリント

「A SMAP Gene Family Encoding ARF GTPase-Activating Proteins and Its Implication in Membrane Trafficking」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル