A simple method for amino acid selective isotope labeling of recombinant proteins in E. coli

Kit I. Tong, Masayuki Yamamoto, Toshiyuki Tanaka

研究成果: Article査読

49 被引用数 (Scopus)

抄録

A simple and user-friendly method of labeling protein selectively with amino acids in vivo is introduced. This technique does not require the use of transaminase-deficient or auxotrophic strains. By manipulating the product feedback inhibitory loops of the E. coli amino acid metabolic pathways and, if necessary, by using enzyme inhibitors, proteins were labeled efficiently in vivo even with amino acid types that are central to the metabolic pathways, such as glutamine. The sequential backbone resonance assignment of the Neh2 domain of Nrf2 transcriptional factor, an intrinsically disordered protein with high spectral degeneracy, was achieved using this labeling method.

本文言語English
ページ(範囲)59-67
ページ数9
ジャーナルJournal of biomolecular NMR
42
1
DOI
出版ステータスPublished - 2008

ASJC Scopus subject areas

  • 生化学
  • 分光学

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