A simple and rapid method for the analysis of fish histamine by paper electrophoresis

Minoru Sato; Zhi Hua Tao; Kazuhiro Shiozaki; Toshiki Nakano; Toshiyasu Yamaguchi; Takehiko Yokoyama; Nobuhiro Kan-No; Eizoh Nagahisa

doi:10.1111/j.1444-2906.2006.01232.x

A simple and rapid method for the analysis of fish histamine by paper electrophoresis

Minoru Sato, Zhi Hua Tao, Kazuhiro Shiozaki, Toshiki Nakano, Toshiyasu Yamaguchi, Takehiko Yokoyama, Nobuhiro Kan-No, Eizoh Nagahisa

農学研究科・資源生物科学専攻

研究成果: Article › 査読

20 被引用数 (Scopus)

抄録

The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly's reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g).

本文言語	English
ページ（範囲）	889-892
ページ数	4
ジャーナル	Fisheries Science
巻	72
号	4
DOI	https://doi.org/10.1111/j.1444-2906.2006.01232.x
出版ステータス	Published - 2006 8

ASJC Scopus subject areas

水圏科学

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10.1111/j.1444-2906.2006.01232.x

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title = "A simple and rapid method for the analysis of fish histamine by paper electrophoresis",

abstract = "The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly's reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g).",

keywords = "Determination, Fish, Histamine, Paper electrophoresis, Pauly reagent",

author = "Minoru Sato and Tao, {Zhi Hua} and Kazuhiro Shiozaki and Toshiki Nakano and Toshiyasu Yamaguchi and Takehiko Yokoyama and Nobuhiro Kan-No and Eizoh Nagahisa",

note = "Funding Information: This work was partly supported by a Grant-in-Aid for Science Research (B) (No. 09556045) from the Ministry of Education, Science, Sports, and Culture of Japan.",

year = "2006",

month = aug,

doi = "10.1111/j.1444-2906.2006.01232.x",

language = "English",

volume = "72",

pages = "889--892",

journal = "Fisheries Science",

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TY - JOUR

T1 - A simple and rapid method for the analysis of fish histamine by paper electrophoresis

AU - Sato, Minoru

AU - Tao, Zhi Hua

AU - Shiozaki, Kazuhiro

AU - Nakano, Toshiki

AU - Yamaguchi, Toshiyasu

AU - Yokoyama, Takehiko

AU - Kan-No, Nobuhiro

AU - Nagahisa, Eizoh

N1 - Funding Information: This work was partly supported by a Grant-in-Aid for Science Research (B) (No. 09556045) from the Ministry of Education, Science, Sports, and Culture of Japan.

PY - 2006/8

Y1 - 2006/8

N2 - The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly's reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g).

AB - The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly's reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g).

KW - Determination

KW - Fish

KW - Histamine

KW - Paper electrophoresis

KW - Pauly reagent

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DO - 10.1111/j.1444-2906.2006.01232.x

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VL - 72

SP - 889

EP - 892

JO - Fisheries Science

JF - Fisheries Science

SN - 0919-9268

IS - 4

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A simple and rapid method for the analysis of fish histamine by paper electrophoresis

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