A screening method for DNA aptamers that bind to a specific, unidentified protein in tissue samples

Takahisa Noma, Kazunori Ikebukuro, Koji Sode, Takuya Ohkubo, Yuji Sakasegawa, Naomi Hachiya, Kiyotoshi Kaneko

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Aptamers are oligonucleotide ligands with a high affinity to, and specificity for, various target molecules and they are expected to be powerful tools for proteomic analysis. To select aptamers that bind to a specific unidentified protein in tissues for protein analysis, a screening method was developed using chicken skeletal muscle as a model. Target proteins in the target mixture were separated by electrophoresis and transferred to a membrane, and a DNA library was added onto it. The aptamers that bound to the target protein were visualized by chemiluminescence and collected by cutting out the visualized band. The specific aptamers to the target protein were selected by only one round of selection using this screening, suggesting this screening method might be useful for selecting aptamers for proteome analysis.

本文言語English
ページ(範囲)1377-1381
ページ数5
ジャーナルBiotechnology Letters
28
17
DOI
出版ステータスPublished - 2006 9

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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