TY - JOUR
T1 - A pyrazine-based fluorescence-enhancing ligand with a high selectivity for thymine in AP site-containing DNA duplexes
AU - Zhao, Chunxia
AU - Rajendran, Arivazhagan
AU - Dai, Qing
AU - Nishizawa, Seiichi
AU - Teramae, Norio
N1 - Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2008/6
Y1 - 2008/6
N2 - A fluorescent pyrazine derivative, 3,5-diamino-6-chloro-2-pyrazine carbonitrile (DCPC), is presented as a promising light-up ligand for single-nucleotide polymorphisms (SNPs) typing. In solutions buffered to pH 7.0 (I = 0.11 M, at 5°C), DCPC can bind to thymine selectively over other nucleobases opposite an abasic site in DNA duplexes (5′-GTGTG CGTTG ANA TGGAC GCAGA-3′/3′-CACAC GCAAC TXT ACCTG CGTCT-5′, X = abasic site, N = target nucleotide) with a dissociation constant of 2.6 μM. The binding of DCPC is accompanied by a significant enhancement of its fluorescence (λ max , 412 nm), and the response is highly selective to thymine base. These binding and sensing properties allow a clear detection of thymine-related mutations present in polymerase chain reaction (PCR) amplification products. 2008
AB - A fluorescent pyrazine derivative, 3,5-diamino-6-chloro-2-pyrazine carbonitrile (DCPC), is presented as a promising light-up ligand for single-nucleotide polymorphisms (SNPs) typing. In solutions buffered to pH 7.0 (I = 0.11 M, at 5°C), DCPC can bind to thymine selectively over other nucleobases opposite an abasic site in DNA duplexes (5′-GTGTG CGTTG ANA TGGAC GCAGA-3′/3′-CACAC GCAAC TXT ACCTG CGTCT-5′, X = abasic site, N = target nucleotide) with a dissociation constant of 2.6 μM. The binding of DCPC is accompanied by a significant enhancement of its fluorescence (λ max , 412 nm), and the response is highly selective to thymine base. These binding and sensing properties allow a clear detection of thymine-related mutations present in polymerase chain reaction (PCR) amplification products. 2008
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U2 - 10.2116/analsci.24.693
DO - 10.2116/analsci.24.693
M3 - Article
C2 - 18544854
AN - SCOPUS:49749085185
VL - 24
SP - 693
EP - 695
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 6
ER -