A pyrazine-based fluorescence-enhancing ligand with a high selectivity for thymine in AP site-containing DNA duplexes

Chunxia Zhao, Arivazhagan Rajendran, Qing Dai, Seiichi Nishizawa, Norio Teramae

研究成果: Article査読

21 被引用数 (Scopus)

抄録

A fluorescent pyrazine derivative, 3,5-diamino-6-chloro-2-pyrazine carbonitrile (DCPC), is presented as a promising light-up ligand for single-nucleotide polymorphisms (SNPs) typing. In solutions buffered to pH 7.0 (I = 0.11 M, at 5°C), DCPC can bind to thymine selectively over other nucleobases opposite an abasic site in DNA duplexes (5′-GTGTG CGTTG ANA TGGAC GCAGA-3′/3′-CACAC GCAAC TXT ACCTG CGTCT-5′, X = abasic site, N = target nucleotide) with a dissociation constant of 2.6 μM. The binding of DCPC is accompanied by a significant enhancement of its fluorescence (λ max , 412 nm), and the response is highly selective to thymine base. These binding and sensing properties allow a clear detection of thymine-related mutations present in polymerase chain reaction (PCR) amplification products. 2008

本文言語English
ページ(範囲)693-695
ページ数3
ジャーナルanalytical sciences
24
6
DOI
出版ステータスPublished - 2008 6

ASJC Scopus subject areas

  • Analytical Chemistry

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