A pollen coat-inducible autoinhibited Ca2+-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the brassicaceae

Megumi Iwano, Motoko Igarashi, Yoshiaki Tarutani, Pulla Kaothien-Nakayama, Hideki Nakayama, Hideki Moriyama, Ryo Yakabe, Tetsuyuki Entani, Hiroko Shimosato-Asano, Masao Ueki, Gen Tamiya, Seiji Takayama

    研究成果: Article査読

    36 被引用数 (Scopus)

    抄録

    In the Brassicaceae, intraspecific non-self pollen (compatible pollen) can germinate and grow into stigmatic papilla cells, while self-pollen or interspecific pollen is rejected at this stage. However, the mechanisms underlying this selective acceptance of compatible pollen remain unclear. Here, using a cell-impermeant calcium indicator, we showed that the compatible pollen coat contains signaling molecules that stimulate Ca2+ export from the papilla cells. Transcriptome analyses of stigmas suggested that autoinhibited Ca2+-ATPase13 (ACA13) was induced after both compatible pollination and compatible pollen coat treatment. A complementation test using a yeast Saccharomyces cerevisiae strain lacking major Ca2+ transport systems suggested that ACA13 indeed functions as an autoinhibited Ca2+ transporter. ACA13 transcription increased in papilla cells and in transmitting tracts after pollination. ACA13 protein localized to the plasma membrane and to vesicles near the Golgi body and accumulated at the pollen tube penetration site after pollination. The stigma of a T-DNA insertion line of ACA13 exhibited reduced Ca2+ export, as well as defects in compatible pollen germination and seed production. These findings suggest that stigmatic ACA13 functions in the export of Ca2+ to the compatible pollen tube, which promotes successful fertilization.

    本文言語English
    ページ(範囲)636-649
    ページ数14
    ジャーナルPlant Cell
    26
    2
    DOI
    出版ステータスPublished - 2014 2

    ASJC Scopus subject areas

    • Plant Science
    • Cell Biology

    フィンガープリント 「A pollen coat-inducible autoinhibited Ca<sup>2+</sup>-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the brassicaceae」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル