TY - JOUR
T1 - A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm
AU - Sukegawa, Jun
AU - Blobel, Günter
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1993/1/15
Y1 - 1993/1/15
N2 - We have molecularly cloned and sequenced a cDNA for a rat liver nucleoporin with a molecular mass of 152.8 kd, termed nup153, that shares a repetitive degenerate pentapeptide motif with a subgroup of nucleoporins of yeast and vertebrates. However, its most striking feature is a novel 4-fold repeat of a Cys2-Cys2-type zinc finger motif. When expressed in E. coli, the zinc finger domain of nup153 binds DNA in a zinc-dependent fashion. Immunoelectron microscopy localized nup153 exclusively to the nucleoplasmic side of the nuclear pore complex. We suggest that nup153 recognizes a specific DNA sequence to organize the genome three-dimensionally and to gate transcribable genes to nuclear pore complexes.
AB - We have molecularly cloned and sequenced a cDNA for a rat liver nucleoporin with a molecular mass of 152.8 kd, termed nup153, that shares a repetitive degenerate pentapeptide motif with a subgroup of nucleoporins of yeast and vertebrates. However, its most striking feature is a novel 4-fold repeat of a Cys2-Cys2-type zinc finger motif. When expressed in E. coli, the zinc finger domain of nup153 binds DNA in a zinc-dependent fashion. Immunoelectron microscopy localized nup153 exclusively to the nucleoplasmic side of the nuclear pore complex. We suggest that nup153 recognizes a specific DNA sequence to organize the genome three-dimensionally and to gate transcribable genes to nuclear pore complexes.
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U2 - 10.1016/0092-8674(93)90047-T
DO - 10.1016/0092-8674(93)90047-T
M3 - Article
C2 - 8422679
AN - SCOPUS:0027458374
VL - 72
SP - 29
EP - 38
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -