A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm

Jun Sukegawa, Günter Blobel

研究成果: Article査読

242 被引用数 (Scopus)

抄録

We have molecularly cloned and sequenced a cDNA for a rat liver nucleoporin with a molecular mass of 152.8 kd, termed nup153, that shares a repetitive degenerate pentapeptide motif with a subgroup of nucleoporins of yeast and vertebrates. However, its most striking feature is a novel 4-fold repeat of a Cys2-Cys2-type zinc finger motif. When expressed in E. coli, the zinc finger domain of nup153 binds DNA in a zinc-dependent fashion. Immunoelectron microscopy localized nup153 exclusively to the nucleoplasmic side of the nuclear pore complex. We suggest that nup153 recognizes a specific DNA sequence to organize the genome three-dimensionally and to gate transcribable genes to nuclear pore complexes.

本文言語English
ページ(範囲)29-38
ページ数10
ジャーナルCell
72
1
DOI
出版ステータスPublished - 1993 1月 15

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル