A novel reporter rat strain that conditionally expresses the bright red fluorescent protein tdTomato

Hiroyuki Igarashi, Kyo Koizumi, Ryosuke Kaneko, Keiko Ikeda, Ryo Egawa, Yuchio Yanagawa, Shin Ichi Muramatsu, Hiroshi Onimaru, Toru Ishizuka, Hiromu Yawo

    研究成果: Article

    8 引用 (Scopus)

    抜粋

    Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato) in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including their projection fibers and spines without any histological enhancement. In addition, a transgenic rat line (FLAME) that ubiquitously expresses tdTomato was successfully established by injecting intracytoplasmic Cre mRNA into fertilized ova. Our rat reporter system will facilitate connectome studies as well as the visualization of the fine structures of genetically identified cells for long periods both in vivo and ex vivo. Furthermore, FLAME is an ideal model for organ transplantation research owing to improved traceability of cells/tissues.

    元の言語English
    記事番号e0155687
    ジャーナルPloS one
    11
    発行部数5
    DOI
    出版物ステータスPublished - 2016 5 1

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

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  • これを引用

    Igarashi, H., Koizumi, K., Kaneko, R., Ikeda, K., Egawa, R., Yanagawa, Y., Muramatsu, S. I., Onimaru, H., Ishizuka, T., & Yawo, H. (2016). A novel reporter rat strain that conditionally expresses the bright red fluorescent protein tdTomato. PloS one, 11(5), [e0155687]. https://doi.org/10.1371/journal.pone.0155687