A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination

Shunsuke Kawamoto, Hitoshi Niwa, Fumi Tashiro, Shigetoshi Sano, Gen Kondoh, Junji Takeda, Koichi Tabayashi, Jun Ichi Miyazaki

研究成果: Article査読

286 被引用数 (Scopus)

抄録

The success of Cre-mediated conditional gene targeting depends on the specificity of Cre recombinase expression in Cre-transgenic mouse lines. As a tool to evaluate the specificity of Cre expression, we developed a reporter transgenic mouse strain that expresses enhanced green fluorescent protein (EGFP) upon Cre-mediated recombination. We demonstrate that the progeny resulting from a cross between this reporter strain and a transgenic strain expressing Cre in zygotes show ubiquitous EGFP fluorescence. This reporter strain should be useful to monitor the Cre expression directed by various promoters in transgenic mice, including mice in which Cre is expressed transiently during embryogenesis under a developmentally regulated promoter. Copyright (C) 2000 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)263-268
ページ数6
ジャーナルFEBS Letters
470
3
DOI
出版ステータスPublished - 2000 3月 31
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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