A novel plant cysteine-rich peptide family conferring cadmium tolerance to yeast and plants

Taiki Matsuda; Masato Kuramata; Yoshihiro Takahashi; Etsuko Kitagawa; Shohab Youssefian; Tomonobu Kusano

doi:10.4161/psb.4.5.8272

A novel plant cysteine-rich peptide family conferring cadmium tolerance to yeast and plants

Taiki Matsuda, Masato Kuramata, Yoshihiro Takahashi, Etsuko Kitagawa, Shohab Youssefian, Tomonobu Kusano

研究成果: Article › 査読

本文言語	English
ページ（範囲）	419-421
ページ数	3
ジャーナル	Plant Signaling and Behavior
巻	4
号	5
DOI	https://doi.org/10.4161/psb.4.5.8272
出版ステータス	Published - 2009 5月
外部発表	はい

ASJC Scopus subject areas

植物科学

文献へのアクセス

10.4161/psb.4.5.8272

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title = "A novel plant cysteine-rich peptide family conferring cadmium tolerance to yeast and plants",

keywords = "Cadmium, Cysteine-rich peptide, Hypoaccumulation, Phytoremediation, Tolerance",

author = "Taiki Matsuda and Masato Kuramata and Yoshihiro Takahashi and Etsuko Kitagawa and Shohab Youssefian and Tomonobu Kusano",

note = "Funding Information: dicotyledonous plants also possess homologous gene(s). It appears Induced expression of DcCDT1 in 109Cd-preloaded cells may well that they do since we found two other examples, a Populus EST allow us to distinguish between these two possibilities. clone (accession number CU225257) and also a Brassica rapa A final question that needs to be addressed is whether the subsp. pekinensis clone (accession number AC189268), that genes encoding this family of proteins can be potentially useful encode DcCDT1 homologues. However, further analyses will be genetic resources for phytoremediation. OsCDT1, one of the five required to determine whether these plants carry a single gene like rice DcCDT1 homologues, reduces the Cd contents of yeast and Arabidopsis or a small gene family as in rice plants. Arabidopsis cells. Therefore, from the viewpoint of food safety, this Several different plant components contribute to heavy metal protein family may be useful since constitutive root-specific expres-homeostasis and detoxification. Among these are the low molecular sion of the genes may contribute to reduced Cd accumulation mass (4–14 kDa) metallothioneins that contain a high ratio of Cys in the edible plant parts. Alternatively, silencing of all DcCDT1 residues, and the small peptides known as phytochelatins that have homologues in rice could plausibly result in the hyperaccumulation the general structure, (γ-Glu-Cys)n-Gly.7-10BOthieor Csysc-riiche pnlanct eo.f Cd and the use of these plants in phytoremediation strategies. proteins involved in Cd tolerance and detoxification have also Acknowledgements been reported.11,12 However, compared to all these, DcCDT1 and We thank Dr. Dennis J. Thiele (Duke University Medical its homologues are unique and distinctive in both their peptide Center) for kindly providing the yeast strains and vector. This work lengths (49–60 amino acids) and arrangement of Cys residues in was supported, in part, by a Grant-in-aid (Hazardous Chemicals) (CLDCL or delete)-CCCC consensus sequence.the CL-(Y/F)-A-(C/T)-X5-CC-(F/C)-CCYE-(T/K)-C-(E/K)-C- from the Ministry of Agriculture, Forestry, and Fisheries of Japan",

year = "2009",

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doi = "10.4161/psb.4.5.8272",

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T1 - A novel plant cysteine-rich peptide family conferring cadmium tolerance to yeast and plants

AU - Matsuda, Taiki

AU - Kuramata, Masato

AU - Takahashi, Yoshihiro

AU - Kitagawa, Etsuko

AU - Youssefian, Shohab

AU - Kusano, Tomonobu

N1 - Funding Information: dicotyledonous plants also possess homologous gene(s). It appears Induced expression of DcCDT1 in 109Cd-preloaded cells may well that they do since we found two other examples, a Populus EST allow us to distinguish between these two possibilities. clone (accession number CU225257) and also a Brassica rapa A final question that needs to be addressed is whether the subsp. pekinensis clone (accession number AC189268), that genes encoding this family of proteins can be potentially useful encode DcCDT1 homologues. However, further analyses will be genetic resources for phytoremediation. OsCDT1, one of the five required to determine whether these plants carry a single gene like rice DcCDT1 homologues, reduces the Cd contents of yeast and Arabidopsis or a small gene family as in rice plants. Arabidopsis cells. Therefore, from the viewpoint of food safety, this Several different plant components contribute to heavy metal protein family may be useful since constitutive root-specific expres-homeostasis and detoxification. Among these are the low molecular sion of the genes may contribute to reduced Cd accumulation mass (4–14 kDa) metallothioneins that contain a high ratio of Cys in the edible plant parts. Alternatively, silencing of all DcCDT1 residues, and the small peptides known as phytochelatins that have homologues in rice could plausibly result in the hyperaccumulation the general structure, (γ-Glu-Cys)n-Gly.7-10BOthieor Csysc-riiche pnlanct eo.f Cd and the use of these plants in phytoremediation strategies. proteins involved in Cd tolerance and detoxification have also Acknowledgements been reported.11,12 However, compared to all these, DcCDT1 and We thank Dr. Dennis J. Thiele (Duke University Medical its homologues are unique and distinctive in both their peptide Center) for kindly providing the yeast strains and vector. This work lengths (49–60 amino acids) and arrangement of Cys residues in was supported, in part, by a Grant-in-aid (Hazardous Chemicals) (CLDCL or delete)-CCCC consensus sequence.the CL-(Y/F)-A-(C/T)-X5-CC-(F/C)-CCYE-(T/K)-C-(E/K)-C- from the Ministry of Agriculture, Forestry, and Fisheries of Japan

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KW - Cadmium

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