A novel method of vesicle preparation by simple dilution of bicelle solution

Shogo Taguchi, Bong Su Kang, Keishi Suga, Yukihiro Okamoto, Ho Sup Jung, Hiroshi Umakoshi

研究成果: Article査読

6 被引用数 (Scopus)


We prepared phospholipid vesicles in continuous dilution from small phospholipid bilayer bicelles. As vesicles are useful as delivery carriers, it is necessary to develop a method of continuous encapsulation. Bicelles are composed of long-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine in bilayers and short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine as a detergent. Bicelles were diluted in a batch system and a flow system and their membrane properties were compared; sizes were analyzed by dynamic light scattering and membrane properties by fluorescence spectroscopy. In the flow system, the initial bicelles (10−20 nm) coalesced by dilution and hydration of the detergent into large vesicles (>30 nm). Uptake of methylene blue solution revealed the internal aqueous phase. Transmission electron microscopy confirmed the formation of vesicles by dilution. The membrane fluidity of the vesicles showed an ordered gel phase. The results suggest that bicelles can form vesicles by continuous dilution. This method is suitable for encapsulating materials.

ジャーナルBiochemical Engineering Journal
出版ステータスPublished - 2020 10月 15

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 環境工学
  • 生体医工学


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