A novel automated microchip flow-chamber system to quantitatively evaluate thrombus formation and antithrombotic agents under blood flow conditions

K. Hosokawa, T. Ohnishi, T. Kondo, M. Fukasawa, T. Koide, I. Maruyama, K. A. Tanaka

研究成果: Article査読

94 被引用数 (Scopus)

抄録

Background and aims:In the present study, we describe a newly developed microchip-based analytical system to evaluate white thrombus formation (WTF). Efficacies of various antithrombotic agents were compared under different flow conditions. Methods:Whole blood containing corn trypsin inhibitor was perfused over a microchip coated with collagen and tissue thromboplastin at the lower and higher shear rates of 240 and 600s -1, and WTF process inside the microchip was quantified by monitoring a flow pressure. Parameters of T 10 (time to 10kPa), T 10-80 (time from 10 to 80kPa) and OT (occlusion time; time to 80kPa) were used to evaluate the onset and the growth rate of WTF, and the capillary occlusion, respectively. Results:After perfusion was started, white thrombus composed of activated platelets and fibrin was formed on the coated surface. Thrombus gradually increased in size and eventually occluded the capillary. Among anticoagulants, heparin (0.5-1.0UmL -1) potently prolonged T 10 at both shear rates, whereas low molecular weight heparin (1.0-2.0IUmL -1) inhibited the growth of WTF at the lower shear rate. Among antiplatelet agents, abciximab (1-2μgmL -1) significantly reduced the size and number of thrombi, which was additively enhanced in the presence of heparin (0.5UmL -1). OS-1 (specific GPIbα-antagonist) prevented the complete capillary occlusion. Conclusion:The novel monitoring system of WTF may be useful in preclinical and clinical evaluations of different types of antithrombotic strategies, and their effects in combination.

本文言語English
ページ(範囲)2029-2037
ページ数9
ジャーナルJournal of Thrombosis and Haemostasis
9
10
DOI
出版ステータスPublished - 2011 10
外部発表はい

ASJC Scopus subject areas

  • 血液学

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