A novel analytical method to evaluate directly catalase activity of microorganisms and mammalian cells by ESR oximetry

Keisuke Nakamura, Taro Kanno, Takayuki Mokudai, Atsuo Iwasawa, Yoshimi Niwano, Masahiro Kohno

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Electron spin resonance (ESR) oximetry technique was applied for analysis of catalase activity in the present study. Catalase activity was evaluated by measuring oxygen from the reaction between hydrogen peroxide (H 2O2) and catalase-positive cells. It was demonstrated that the ESR spectra of spin-label probes, 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL), 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (4-oxo-TEMPO) and 4-maleimido-2,2,6,6-tetramethyl-1-piperidinyloxy (4-maleimido-TEMPO) in the presence of H2O2 were broadened with the concentrations of catalase. It was possible to make a calibration curve for catalase activity by peak widths of the spectra of each spin-label probe, which are broadened dependently on catalase concentrations. The broadened ESR spectra were also observed when the catalase-positive micro-organisms or the mammalian cells originally from circulating monocytes/macrophages were mixed with TEMPOL and H2O2. Meanwhile, catalase-negative micro-organisms caused no broadening change of ESR spectra. The present study indicates that it is possible to evaluate directly the catalase activity of various micro-organisms and mammalian cells by using an ESR oximetry technique.

本文言語English
ページ(範囲)1036-1043
ページ数8
ジャーナルFree Radical Research
44
9
DOI
出版ステータスPublished - 2010 9

ASJC Scopus subject areas

  • 生化学

フィンガープリント

「A novel analytical method to evaluate directly catalase activity of microorganisms and mammalian cells by ESR oximetry」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル