A newly established megakaryoblastic/erythroid cell line that differentiates to red cells in the presence of erythropoietin and produces platelet‐like particles

Reiko Tsuyuoka, Takayuki Takahashi, Akira Suzuki, Yutaka Sasaki, Kishiko Nakamura, Manabu Fukumoto, Katsuyuki Ohmori, Yohichiro Ohno, Kazuwa Nakao

研究成果: Article査読

13 被引用数 (Scopus)

抄録

In August, 1992, we established a leukemic cell line (NS‐Meg) from a patient in megakaryoblastic transformation of Philadelphia chromosome‐positive chronic myeloid leukemia. The NS‐Meg cells were positive for α‐naphthyl acetate esterase and periodic acid‐Schiff (PAS) staining and for surface CD4, CD7, CD13, CD34, CD41a, and glycophorin A antigens. Ultrastructurally, the cells had α‐granules, demarcation membranes, and platelet peroxidase activity. The NS‐Meg cells spontaneously produced platelet‐like particles which contained α‐granules, mitochondria and dense bodles, strongly suggesting platelet production. Erythropoietin (Epo), granulocyte/macrophage colony stimulating factor(GM‐CSF), and interleukin 3 (IL‐3) promoted the growth of NS‐Meg cells. Phorbol‐12‐myristate‐13‐acetate increased the expression of both CD41a and CD61 antigens. Ten‐day exposure to Epo induced mature erythroblasts and red cells. These benzidine‐positive cells were positive for hemoglobin F staining. Untreated NS‐Meg cells expressed mRNA for the Epo receptor (EpoR), for GATA‐1, and for α1, α2 and γ globin genes. These results indicate that NS‐Meg cells undergo terminal differentiation of both megakaryocyte and erythroid lineages. This cell line should be a very useful tool for the investigation of both megakaryocytic and erythroid maturation.

本文言語English
ページ(範囲)54-64
ページ数11
ジャーナルStem Cells
13
1
DOI
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 分子医療
  • 発生生物学
  • 細胞生物学

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