抄録
A hemagglutination (HA) assay was done for the screening of lectin-like components in surface layer protein (SLP) from Lactobacillus (L.) acidophilus A group strains. The new screening method, using polystyrene beads coated with rat-colonic mucin (RCM), which combines sugar chains similar to those of human colonic mucin, was also done. The results showed that the HA assay was not a good indicator for selecting strains having high adhesion to the human intestinal tract. The SLPs from 3 strains that strongly bound to RCM also bound well to carbohydrate portions of Carnoy’s-fixed human colonic mucous layer. These results suggest that this method is a new promising screening technique for the L. acidophilus strains having high adhesion to the human intestinal tract.
本文言語 | English |
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ページ(範囲) | 1434-1438 |
ページ数 | 5 |
ジャーナル | Bioscience, Biotechnology and Biochemistry |
巻 | 60 |
号 | 9 |
DOI | |
出版ステータス | Published - 1996 1 |
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry