A new decalcifying technique for immunohistochemical studies of calcified tissue, especially applicable to cell surface marker demonstration

S. Mori, T. Sawai, T. Teshima, M. Kyogoku

研究成果: Article査読

64 被引用数 (Scopus)

抄録

We have developed a new decalcifying technique for use in light and electron microscopy studies utilizing immunohistochemical staining of calcified tissues. Specimens containing calcified tissues can be adequately decalcified at a pH of 7.1-7.4 and temperature of -5°C, without freezing, by use of a solution containing EDTA, sodium hydroxide, and glycerol. In this study, Leu-2a, Leu-3a, Leu-4, Leu-7, Leu-12, Leu-14, Leu-M1, Leu-M2, Leu-M3, and HLA-DR-positive cells in destructive lesions of bone tissues from patients with rheumatoid arthritis and osteomyelitis were successfully detected immunohistochemically. Furthermore, the presence of HLA-DR antigen on the surface of the infiltrating cells in the same lesions could be demonstrated using the immunoelectron microscopic technique. The results reported here have not previously been obtainable using conventional decalcifying techniques.

本文言語English
ページ(範囲)111-114
ページ数4
ジャーナルJournal of Histochemistry and Cytochemistry
36
1
DOI
出版ステータスPublished - 1988

ASJC Scopus subject areas

  • Anatomy
  • Histology

フィンガープリント 「A new decalcifying technique for immunohistochemical studies of calcified tissue, especially applicable to cell surface marker demonstration」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル