A new assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilus group to human colonic mucin

Hideaki Uchida; Kenji Fujitani; Yasushi Kawai; Haruki Kitazawa; Akira Horii; Kenichi Shiiba; Kazuya Saito; Tadao Saito

doi:10.1271/bbb.68.1004

A new assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilus group to human colonic mucin

Hideaki Uchida, Kenji Fujitani, Yasushi Kawai, Haruki Kitazawa, Akira Horii, Kenichi Shiiba, Kazuya Saito, Tadao Saito

研究成果: Article › 査読

45 被引用数 (Scopus)

抄録

A new binding assay to investigate the mechanism of adhesion of lactic acid bacteria to the human intestine was established by the surface plasmon resonance technique using a biosensor BIACORE1000. Cells of 26 strains of the Lactobacillus acidophilus group as analytes were eluted onto a sensor chip on which were immobilized biotinylated A-trisaccharide polymer probes having human A-type antigen [(GalNAcα1-3(Fucα1-2)Gal)-] or human colonic mucin of blood type A (HCM-A) as ligands. In the first screening, high adhesive affinity to the A-trisaccharide BP-probe was observed in L. acidophilus OLL2769, L. crispatus JCM8778, LA205 and LA206. In the second screening, which used HCM-A, only L. acidophilus OLL2769 and L. crispatus JCM8778 were selected as adhesive strains with specific binding ability to human A-antigen. The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM.

本文言語	English
ページ（範囲）	1004-1010
ページ数	7
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	68
号	5
DOI	https://doi.org/10.1271/bbb.68.1004
出版ステータス	Published - 2004 5月

ASJC Scopus subject areas

バイオテクノロジー
分析化学
生化学
応用微生物学とバイオテクノロジー
分子生物学
有機化学

文献へのアクセス

10.1271/bbb.68.1004

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引用スタイル

A new assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilus group to human colonic mucin. / Uchida, Hideaki; Fujitani, Kenji; Kawai, Yasushi; Kitazawa, Haruki; Horii, Akira; Shiiba, Kenichi; Saito, Kazuya; Saito, Tadao.

In: Bioscience, Biotechnology and Biochemistry, Vol. 68, No. 5, 05.2004, p. 1004-1010.

研究成果: Article › 査読

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abstract = "A new binding assay to investigate the mechanism of adhesion of lactic acid bacteria to the human intestine was established by the surface plasmon resonance technique using a biosensor BIACORE1000. Cells of 26 strains of the Lactobacillus acidophilus group as analytes were eluted onto a sensor chip on which were immobilized biotinylated A-trisaccharide polymer probes having human A-type antigen [(GalNAcα1-3(Fucα1-2)Gal)-] or human colonic mucin of blood type A (HCM-A) as ligands. In the first screening, high adhesive affinity to the A-trisaccharide BP-probe was observed in L. acidophilus OLL2769, L. crispatus JCM8778, LA205 and LA206. In the second screening, which used HCM-A, only L. acidophilus OLL2769 and L. crispatus JCM8778 were selected as adhesive strains with specific binding ability to human A-antigen. The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM.",

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author = "Hideaki Uchida and Kenji Fujitani and Yasushi Kawai and Haruki Kitazawa and Akira Horii and Kenichi Shiiba and Kazuya Saito and Tadao Saito",

note = "Funding Information: This work was partially supported by a Grant-in-Aid for Scientific Research (B) (2) (No. 10660254, 12460117) from the Japan Society for the Promotion of Science (JSPS) and the Food Science Institute Foundation (Ryoshoku-Kenkyukai). We thank to Meiji Dairies Corporation (Tokyo, Japan) for gifting L. acidophilus OLL2769 strain.",

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N2 - A new binding assay to investigate the mechanism of adhesion of lactic acid bacteria to the human intestine was established by the surface plasmon resonance technique using a biosensor BIACORE1000. Cells of 26 strains of the Lactobacillus acidophilus group as analytes were eluted onto a sensor chip on which were immobilized biotinylated A-trisaccharide polymer probes having human A-type antigen [(GalNAcα1-3(Fucα1-2)Gal)-] or human colonic mucin of blood type A (HCM-A) as ligands. In the first screening, high adhesive affinity to the A-trisaccharide BP-probe was observed in L. acidophilus OLL2769, L. crispatus JCM8778, LA205 and LA206. In the second screening, which used HCM-A, only L. acidophilus OLL2769 and L. crispatus JCM8778 were selected as adhesive strains with specific binding ability to human A-antigen. The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM.

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