抄録
A new binding assay to investigate the mechanism of adhesion of lactic acid bacteria to the human intestine was established by the surface plasmon resonance technique using a biosensor BIACORE1000. Cells of 26 strains of the Lactobacillus acidophilus group as analytes were eluted onto a sensor chip on which were immobilized biotinylated A-trisaccharide polymer probes having human A-type antigen [(GalNAcα1-3(Fucα1-2)Gal)-] or human colonic mucin of blood type A (HCM-A) as ligands. In the first screening, high adhesive affinity to the A-trisaccharide BP-probe was observed in L. acidophilus OLL2769, L. crispatus JCM8778, LA205 and LA206. In the second screening, which used HCM-A, only L. acidophilus OLL2769 and L. crispatus JCM8778 were selected as adhesive strains with specific binding ability to human A-antigen. The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM.
本文言語 | English |
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ページ(範囲) | 1004-1010 |
ページ数 | 7 |
ジャーナル | Bioscience, Biotechnology and Biochemistry |
巻 | 68 |
号 | 5 |
DOI | |
出版ステータス | Published - 2004 5月 |
ASJC Scopus subject areas
- バイオテクノロジー
- 分析化学
- 生化学
- 応用微生物学とバイオテクノロジー
- 分子生物学
- 有機化学