A millisecond structured illumination microscope for super-resolution live cell imaging

Tomu Suzuki, Shinji Kajimoto, Narufumi Kitamura, Mayumi Takano-Kasuya, Naoko Furusawa, Yasushi Nakano, Hiroshi Fukumura, Kohsuke Gonda, Takakazu Nakabayashi

研究成果: Article査読

1 被引用数 (Scopus)

抄録

In typical super-resolution microscopy, high temporal resolution is sacrificed to achieve a spatial resolution beyond the diffraction limit. In this study, we have developed a new structured illumination microscopy (SIM) system using two Pockels cells, achieving a temporal resolution of 1 ms. The introduction of the two Pockels cells enables control of the phases of the illumination pattern along the X- A nd Y-axes in microseconds. We obtained five images consecutively with different illumination patterns and reconstructed a super-resolution image. We observed fluorescent nanoparticles in living cells for 8 s with super resolution and a frame rate of 1,000 fps.

本文言語English
論文番号045002
ジャーナルApplied Physics Express
13
4
DOI
出版ステータスPublished - 2020 4 1

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

フィンガープリント

「A millisecond structured illumination microscope for super-resolution live cell imaging」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル