A microwell culture system that allows group culture and is compatible with human single media

Shoko Ieda, Tomonori Akai, Yoko Sakaguchi, Sumi Shimamura, Atsushi Sugawara, Masahiro Kaneda, Satoko Matoba, Masanori Kagota, Satoshi Sugimura, Hirotsune Kaijima

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Purpose: A microwell culture system that facilitates group culture, such as well-of-the-well (WOW), improves embryonic development in an individual culture. We examined the effect of WOW on embryonic development in vitro with commercially available human single culture media. Methods: Using four different commercial human single culture media, in vitro development and imprinted gene expression of bovine embryos cultured in WOW were compared to droplet culture (one zygote per drop). To determine the effects of microwell and group culture on embryonic development, different numbers of embryos were cultured in droplet or WOW. Diffusion simulation of accumulating metabolites was conducted using the finite volume method. Results: WOW had a positive effect on bovine embryonic development, regardless of the type of single culture media. Imprinted gene expression was not different between droplet- and WOW-derived blastocysts. The microwell and group cultures in WOW showed a significant positive effect on the rate of total blastocysts and the rate of development to the expanded and hatching blastocyst stages. The assumed cumulative metabolite concentration of WOW with one embryo was 1.47 times higher than that of droplet culture with one embryo. Furthermore, the concentration of WOW with three embryos was 1.54 times higher than that of WOW with one embryo. Conclusions: In using human single culture media, a microwell culture system that allows group culture could be a powerful clinical tool for improving the success of assisted reproductive technologies.

本文言語English
ページ(範囲)1869-1880
ページ数12
ジャーナルJournal of Assisted Reproduction and Genetics
35
10
DOI
出版ステータスPublished - 2018 10月 1
外部発表はい

ASJC Scopus subject areas

  • 生殖医学
  • 遺伝学
  • 産婦人科学
  • 発生生物学
  • 遺伝学(臨床)

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