A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate

Li Liu, Chunxiong Luo, Xiaofeng Ni, Li Wang, Kaori Yamauchi, Shinichi M. Nomura, Norio Nakatsuji, Yong Chen

研究成果: Article査読

18 被引用数 (Scopus)

抄録

We have developed a combined micro-channel and micro-well system for easy cell loading, culture and post-culture operation on a chip. To demonstrate the reliability of the system, on chip cell culture and differentiation were performed with different types of substrates made of culture dish, glass cover slide and polydimethylsiloaxe (PDMS). As expected, mouse embryo fibroblasts (MEF) showed different adhesion and growth rate on different substrates. When embryonic stem (ES) cells were co-cultured with MEFs, the formation of ES colonies is efficient on both glass and Petri dish, although PDMS could also be used. Finally, ES cell differentiation with neuron growth factors was performed on different substrates, showing clear advantages of using culture Petri dish over both glass and PDMS.

本文言語English
ページ(範囲)505-511
ページ数7
ジャーナルBiomedical microdevices
12
3
DOI
出版ステータスPublished - 2010 6月
外部発表はい

ASJC Scopus subject areas

  • 生体医工学
  • 分子生物学

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