A Method for In Vitro Fabrication of Hybrid Bone/Cartilage Tissue Using Mouse Induced Pluripotent Stem Cells

研究成果: Chapter

抄録

In the developing embryo, bone and cartilage share the same progenitors. However, osteo-chondrogenic induction of mouse induced pluripotent stem cells (iPSCs) remains difficult. Here we describe a protocol to guide iPSCs to differentiate into osteochondral cells that form hybrid bone/cartilage constructs in vitro. Single mouse iPSCs are first reaggregated in ultra-low-attachment micro-space culture plates. At day 12, iPSC spheres are subjected to shaking culture and maintained in an osteogenic induction medium for 31 days (Os induction). In another condition, the osteogenic induction medium is replaced by chondrogenic induction medium at day 22 and maintained for a further 21 days (Os-Chon induction). Os induction produced robust mineralization and some cartilage-like tissue, whereas Os-Chon induction resulted in partial mineralization and a large area of cartilage tissue.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ509-520
ページ数12
DOI
出版ステータスPublished - 2022

出版物シリーズ

名前Methods in Molecular Biology
2454
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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