A hydroxysteroid sulfotransferase, St2b2, is a skin cholesterol sulfotransferase in mice

Miki Shimada, Yoshiteru Kamiyama, Akira Sato, Wataru Honma, Kiyoshi Nagata, Yasushi Yamazoe

    研究成果: Article査読

    11 被引用数 (Scopus)

    抄録

    The mRNA of a sulfotransferase (St2b2) mediating cholesterol sulfation was detected in mouse skin. Recombinant St2b2 also mediated the sulfation of pregnenolone, 3β-hydroxy-5-cholen-24-oic acid, and dehydroepiandrosterone. St2b2 protein was detected in skin cytosols on Western blotting. The addition of 10 nM TPA to skin epidermal cells from newborn mice resulted in a twofold increase in cholesterol sulfation and concomitantly enhanced the St2b2 content after 40 h. Other candidate cholesterol sulfotransferases, St2a4 and St2a9, were not detected in skin by RT-PCR. These results indicate that St2b2 is a cholesterol sulfotransferase in mouse skin.

    本文言語English
    ページ(範囲)167-169
    ページ数3
    ジャーナルJournal of biochemistry
    131
    2
    DOI
    出版ステータスPublished - 2002 1 1

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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