A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor

Shun Sawatsubashi, Takuya Murata, Jinseon Lim, Ryoji Fujiki, Saya Ito, Eriko Suzuki, Masahiko Tanabe, Yue Zhao, Shuhei Kimura, Sally Fujiyama, Takashi Ueda, Daiki Umetsu, Takashi Ito, Ken Ichi Takeyama, Shigeaki Kato

研究成果: Article査読

103 被引用数 (Scopus)

抄録

Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary gland, Drosophila DEK (dDEK) was genetically identified as a coactivator of EcR in both insect cells and intact flies. Biochemical purification and characterization of the complexes containing fly and human DEKs revealed that DEKs serve as histone chaperones via phosphorylation by forming complexes with casein kinase 2. Consistent with the preferential association of the DEK complex with histones enriched in active epigenetic marks, dDEK facilitated H3.3 assembly during puff formation. In some human myeloid leukemia patients, DEK was fused to CAN by chromosomal translocation. This mutation significantly reduced formation of the DEK complex, which is required for histone chaperone activity. Thus, the present study suggests that at least one histone chaperone can be categorized as a type of transcriptional coactivator for nuclear receptors.

本文言語English
ページ(範囲)159-170
ページ数12
ジャーナルGenes and Development
24
2
DOI
出版ステータスPublished - 2010 1 15
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 発生生物学

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