A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes

Ryo Takano, Hiroko Kozuka-Hata, Daisuke Kondoh, Hiroki Bochimoto, Masaaki Oyama, Kentaro Kato

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The pathogenesis of malaria parasites depends on host erythrocyte modifications that are facilitated by parasite proteins exported to the host cytoplasm. These exported proteins form a trafficking complex in the host cytoplasm that transports virulence determinants to the erythrocyte surface; this complex is thus essential for malaria virulence. Here, we report a comprehensive interaction network map of this complex. We developed authentic, unbiased, highly sensitive proteomic approaches to determine the proteins that interact with a core component of the complex, SBP1 (skeleton-binding protein 1). SBP1 interactomes revealed numerous exported proteins and potential interactors associated with SBP1 intracellular trafficking. We identified several host-parasite protein interactions and linked the exported protein MAL8P1.4 to Plasmodium falciparum virulence in infected erythrocytes. Our study highlights the complicated interplay between parasite and host proteins in the host cytoplasm and provides an interaction dataset connecting dozens of exported proteins required for P. falciparum virulence. Microbiology; Parasitology; Proteomics

本文言語English
ページ(範囲)703-714
ページ数12
ジャーナルiScience
19
DOI
出版ステータスPublished - 2019 9 27

ASJC Scopus subject areas

  • 一般

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