A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis

Tetsuro Kuga, Motoo Yamasaki, Susumu Sekine, Masanori Fukui, Yoshiharu Yokoo, Seiga Itoh, Mitsuaki Yoshida, Toshio Hattori, Kiyoshi Takatsuki

研究成果: Article査読

16 被引用数 (Scopus)

抄録

A fused gene of the gag and env sequences of human T-cell leukemia virus type I (HTLV-I), the causative agent of adult T-cell leukemia, was constructed in vitro and expressed in Escherichia coli. The gag-env hybrid protein accumulated as insoluble granules with a yield of approximately 12% of the total proteins. In an enzyme-linked immunosorbent assay done with the use of the gag-env hybrid protein, all 57 seropositive sera gave positive signals, and none of the sera from normal persons did. This system can produce large quantities of the gag-env hybrid protein, which can be used for mass screening of human sera for HTLV-I infection.

本文言語English
ページ(範囲)1168-1173
ページ数6
ジャーナルJapanese Journal of Cancer Research
79
11
出版ステータスPublished - 1988 11

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

フィンガープリント

「A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル