A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals

Hiroshi P.M. Horikawa; Hideo Saisu; Toru Ishizuka; Yoko Sekine; Akira Tsugita; Shoji Odani; Teruo Abe

doi:10.1016/0014-5793(93)80281-X

A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals

Hiroshi P.M. Horikawa, Hideo Saisu, Toru Ishizuka, Yoko Sekine, Akira Tsugita, Shoji Odani, Teruo Abe

研究成果: Article › 査読

76 被引用数 (Scopus)

抄録

Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.

本文言語	English
ページ（範囲）	236-240
ページ数	5
ジャーナル	FEBS Letters
巻	330
号	2
DOI	https://doi.org/10.1016/0014-5793(93)80281-X
出版ステータス	Published - 1993 9 13

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

文献へのアクセス

10.1016/0014-5793(93)80281-X

フィンガープリント「A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

@article{02e525ed1e0240c99b99b49eab93edaf,

title = "A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals",

abstract = "Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.",

keywords = "Neurotransmitter release, Rab3A/smg25A, SNAP-25, Syntaxin, VAMP/synaptobrevin-2",

author = "Horikawa, {Hiroshi P.M.} and Hideo Saisu and Toru Ishizuka and Yoko Sekine and Akira Tsugita and Shoji Odani and Teruo Abe",

year = "1993",

month = sep,

day = "13",

doi = "10.1016/0014-5793(93)80281-X",

language = "English",

volume = "330",

pages = "236--240",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals

AU - Horikawa, Hiroshi P.M.

AU - Saisu, Hideo

AU - Ishizuka, Toru

AU - Sekine, Yoko

AU - Tsugita, Akira

AU - Odani, Shoji

AU - Abe, Teruo

PY - 1993/9/13

Y1 - 1993/9/13

N2 - Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.

AB - Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.

KW - Neurotransmitter release

KW - Rab3A/smg25A

KW - SNAP-25

KW - Syntaxin

KW - VAMP/synaptobrevin-2

UR - http://www.scopus.com/inward/record.url?scp=0027194642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027194642&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(93)80281-X

DO - 10.1016/0014-5793(93)80281-X

M3 - Article

C2 - 8365494

AN - SCOPUS:0027194642

VL - 330

SP - 236

EP - 240

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -