A competitive immunochromatographic assay for testosterone based on electrochemical detection

Kumi Inoue, Pascal Ferrante, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

研究成果: Article査読

39 被引用数 (Scopus)

抄録

An immunochromatographic assay using nitrocellulose membrane was combined with electrochemical detection using an electrode chip in order to quantitatively detect testosterone as a model analyte. The electrode chip consisted of a gold working electrode, a counter electrode and a pseudo-reference electrode, all fabricated on the bottom of a 3.2 mm × 3.2 mm well. Competitive immunoreactions on the membrane were initiated by flowing a solution containing testosterone and horseradish peroxidase (HRP)-labeled testosterone (a competitor) over the membrane. Prepared membrane was placed in a solution containing ferrocenemethanol (FcOH) and H2O2 in the well of the electrode chip, and the enzyme reaction was detected by amperometry. Labeled HRP captured on the membrane catalyzed the oxidation of FcOH to the oxidized form FcOH+, which was reduced electrochemically by the electrode chip. The electrochemical response of the reduction current decreased with increasing concentration of testosterone over the range 1-625 ng/ml.

本文言語English
ページ(範囲)886-892
ページ数7
ジャーナルTalanta
73
5
DOI
出版ステータスPublished - 2007 10 31

ASJC Scopus subject areas

  • 分析化学
  • 化学 (全般)
  • 生化学
  • 分光学

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