A cell cycle-dependent co-repressor mediates photoreceptor cell-specific nuclear receptor function

Shinichiro Takezawa, Atsushi Yokoyama, Maiko Okada, Ryoji Fujiki, Aya Iriyama, Yasuo Yanagi, Hiroaki Ito, Ichiro Takada, Masahiko Kishimoto, Atsushi Miyajima, Ken Ichi Takeyama, Kazuhiko Umesono, Hirochika Kitagawa, Shigeaki Kato

研究成果: Article査読

44 被引用数 (Scopus)

抄録

Photoreceptor cell-specific nuclear receptor (PNR) (NR2E3) acts as a sequence-specific repressor that controls neuronal differentiation in the developing retina. We identified a novel PNR co-repressor, Ret-CoR, that is expressed in the developing retina and brain. Biochemical purification of Ret-CoR identified a multiprotein complex that included E2F/Myb-associated proteins, histone deacetylases (HDACs) and NCoR/HDAC complex-related components. Ret-CoR appeared to function as a platform protein for the complex, and interacted with PNR via two CoRNR motifs. Purified Ret-CoR complex exhibited HDAC activity, co-repressed PNR transrepression function in vitro, and co-repressed PNR function in PNR target gene promoters, presumably in the retinal progenitor cells. Notably, the appearance of Ret-CoR protein was cell-cycle-stage-dependent (from G1 to S). Therefore, Ret-CoR appears to act as a component of an HDAC co-repressor complex that supports PNR repression function in the developing retina, and may represent a co-regulator class that supports transcriptional regulator function via cell-cycle-dependent expression.

本文言語English
ページ(範囲)764-774
ページ数11
ジャーナルEMBO Journal
26
3
DOI
出版ステータスPublished - 2007 2 7
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)

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