8-Nitroguanine, a product of nitrative DNA damage caused by reactive nitrogen species: Formation, occurrence, and implications in inflammation and carcinogenesis

Hiroshi Ohshima, Tomohiro Sawa, Takaaki Akaike

研究成果: Review article査読

108 被引用数 (Scopus)

抄録

The authors review studies on 8-nitroguanine (8-NO2-G) formed by reactions of guanine, guanosine, and 2′-deoxyguanosine, either free or in DNA or RNA with reactive nitrogen species (RNS) generated from peroxynitrite, the myeloperoxidase-H2O2-nitrite system, and others. Use of antibodies against 8-NO2-G lias revealed increased formation of 8-NO2-G in various pathological conditions, including RNA virus-induced pneumonia in mice, intrahepatic bile ducts of hamsters infected with the liver fluke Opisthorchis viverrini, and gastric mucosa of patients with Helicobacter pylori-induced gastritis. Immunoreactivity has been found in the cytosol as well as in the nucleus of inflammatory cells and epithelial cells in inflamed tissues, but not in normal tissues. 8-NO2-G in DNA is potentially mutagenic, yielding G:C to T:A transversion, possibly through its rapid depurination to form an apurinic site and/or miscoding with adenine. 8-NO2-G in RNA may interfere with RNA functions and metabolism. Nitrated guanine nucleosides and nucleotides in the nucleotide pool may contribute to oxidative stress via production of superoxide mediated by various reductases and may disturb or modulate directly various important enzymes such as GTP-binding proteins and cGMP-dependent enzymes. Further studies are warranted to establish the roles of 8-NO2-G in various pathopliysiological conditions and inflammation-associated cancer.

本文言語English
ページ(範囲)1033-1045
ページ数13
ジャーナルAntioxidants and Redox Signaling
8
5-6
DOI
出版ステータスPublished - 2006 5月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 分子生物学
  • 臨床生化学
  • 細胞生物学

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