3′-Blocking Damage of DNA as a Mutagenic Lesion Caused by Hydrogen Peroxide in Escherichia coli

Tadashi Takemoto, Qiu Mei Zhang, Yukiko Matsumoto, Seiji Mito, Tadahide Izumi, Hironobu Ikehata, Shuji Yonei

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Ionizing radiation and hydrogen peroxide (H2O2) produce many types of oxidative DNA damage sucn as strand breaks, apurinic/apyrimidinic (AP) sites, base modifications and 3′-blocking damage such as 3′-phosphoglycolated and 3′-phosphorylated termini. AP sites and 3′-blocking damage are repairable by exonuclease III and endonuclease IV in Escherichia coli. XthA-nfo double mutants of E. coli, which are deficient in exonuclease III and endonuclease IV. were highly sensitive to lethal and mutagenic effects ot H2O2, compared with the wild-type strains. The pNT180 and pNT186 plasmids containing wild-type nfo and mutant nfa-186 gene, respectively, were introduced into the xthA-nfo mutant. The nfo-186 gene product. Nfo186. retained normal AP endonuclease activity but could not remove 3′-blocking damage from DNA. The pNT180 corrected the sensitivity of the xthA-nfo mutant to lethal and mutagenic effects of H2O2. On the other hand, the pNT186 did not have any complementation effects. From these results it was concluded that 3′-blocking damage rather than an AP site is the primary lesion responsible for both lethal and mutagenic effects of H2O2.

本文言語English
ページ(範囲)137-144
ページ数8
ジャーナルJournal of radiation research
39
2
DOI
出版ステータスPublished - 1998 6

ASJC Scopus subject areas

  • 放射線
  • 放射線学、核医学およびイメージング
  • 健康、毒物学および変異誘発

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