3β-hydroxysteroid dehydrogenase isoforms in human aldosterone-producing adenoma

Sachiko Konosu-Fukaya, Yasuhiro Nakamura, Fumitoshi Satoh, Saulo J.A. Felizola, Takashi Maekawa, Yoshikiyo Ono, Ryo Morimoto, Kazue Ise, Ken ichiro Takeda, Koshin Katsu, Fumiyoshi Fujishima, Atsuko Kasajima, Mika Watanabe, Yoichi Arai, Elise P. Gomez-Sanchez, Celso E. Gomez-Sanchez, Masao Doi, Hitoshi Okamura, Hironobu Sasano

研究成果: Article査読

28 被引用数 (Scopus)

抄録

It has become important to evaluate the possible involvement of 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) and 2 (HSD3B2) isoforms in aldosterone-producing adenoma (APA). In this study, we studied 67 and 100 APA cases using real-time quantitative PCR (qPCR) and immunohistochemistry, respectively. Results of qPCR analysis demonstrated that HSD3B2 mRNA was significantly more abundant than HSD3B1 mRNA (P < 0.0001), but only HSD3B1 mRNA significantly correlated with CYP11B2 (aldosterone synthase) mRNA (P < 0.0001) and plasma aldosterone concentration (PAC) of the patients (P < 0.0001). Results of immunohistochemistry subsequently revealed that HSD3B2 immunoreactivity was detected in the great majority of APA but a significant correlation was also detected between HSD3B1 and CYP11B2 (P < 0.0001). In KCNJ5 mutated APA, CYP11B2 mRNA (P < 0.0001) and HSD3B1 mRNA (P = 0.011) were significantly higher than those of wild type APA. These results suggest that HSD3B1 is involved in aldosterone production, despite its lower levels of expression compared with HSD3B2, and also possibly associated with KCNJ5 mutation in APA.

本文言語English
ページ(範囲)205-212
ページ数8
ジャーナルMolecular and Cellular Endocrinology
408
DOI
出版ステータスPublished - 2015 6 5

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 内分泌学

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