TY - JOUR
T1 - 2,2′-dihydroxyazobenzene derivatives as reagents for trace metal determination by ion-pair reversed-phase high-performance liquid chromatography with spectrophotometric detection
AU - Hoshino, Hitoshi
AU - Nakanot, Kouji
AU - Yotsuyanagi, Takao
PY - 1990/1/1
Y1 - 1990/1/1
N2 - 2,2′-Dihydroxyazobenzene and five substituted analogues have been examined as pre-column chelating agents for the determination of trace metal ions by reversed-phase high-performance liquid chromatography (RP-HPLC) with spectrophotometric detection. Seven metal ions, viz., AlIII, CoIII, CuIII, FeIII, MoVI, VV and ZnII, were detected as their azo chelates in the ion-pair RP mode. The optimum conditions, including pH for colour formation, column used, mobile phase parameters and detection wavelength, were investigated for each individual reagent. The method was applied to the determination of AI and Fe in coal fly ash samples. The kinetic nature and the forms of the chelates responsible for the selectivity of RP-HPLC towards metal ions are discussed in terms of "kinetic differentiation" together with ion-pair RP partition phenomena.
AB - 2,2′-Dihydroxyazobenzene and five substituted analogues have been examined as pre-column chelating agents for the determination of trace metal ions by reversed-phase high-performance liquid chromatography (RP-HPLC) with spectrophotometric detection. Seven metal ions, viz., AlIII, CoIII, CuIII, FeIII, MoVI, VV and ZnII, were detected as their azo chelates in the ion-pair RP mode. The optimum conditions, including pH for colour formation, column used, mobile phase parameters and detection wavelength, were investigated for each individual reagent. The method was applied to the determination of AI and Fe in coal fly ash samples. The kinetic nature and the forms of the chelates responsible for the selectivity of RP-HPLC towards metal ions are discussed in terms of "kinetic differentiation" together with ion-pair RP partition phenomena.
KW - 2,2′-Dihydroxyazobenzene reagent
KW - Ion-pair reversed-phase high-performance liquid chromatography
KW - Kinetic differentiation
KW - Metal chelate
KW - Spectrophotometry
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U2 - 10.1039/AN9901500133
DO - 10.1039/AN9901500133
M3 - Article
AN - SCOPUS:0025269554
VL - 115
SP - 133
EP - 137
JO - Analyst
JF - Analyst
SN - 0003-2654
IS - 2
ER -