1-Deoxynojirimycin attenuates high glucose-accelerated senescence in human umbilical vein endothelial cells

E. Shuang, Ryo Kijima, Taro Honma, Kazushi Yamamoto, Yu Hatakeyama, Yasuna Kitano, Toshiyuki Kimura, Kiyotaka Nakagawa, Teruo Miyazawa, Tsuyoshi Tsuduki

研究成果: Article査読

26 被引用数 (Scopus)

抄録

The influence of 1-deoxynojirimycin (DNJ) derived from mulberry on senescence of endothelial cells was examined with the goal of discovery of a method for prevention of senescence of blood vessels. The effect of DNJ on senescence of human umbilical vein endothelial cells (HUVECs) promoted under high glucose condition was determined. HUVECs were cultured in normal glucose (5.6. mmol/L, NG group), normal glucose plus DNJ (10. μmol/L, DNJ group), high glucose (30. mmol/L, HG group), or high glucose plus DNJ (10. μmol/L, HG. +. DNJ group) and passaged until they reached senescence. The proliferation rate was markedly decreased in the HG group compared with the NG group, and this phenomenon was reversed by DNJ. The frequency of senescent (SA-ß-Gal-positive) cells and the expression level of senescence genes (PAI-1 and p21) were significantly higher in the HG group compared with the NG group, and these changes were blocked by DNJ. Monocyte adhesion, NF-kB activity, and reactive oxygen species production, all of which promote cellular senescence, were significantly increased in the HG group compared with the NG group, and again these changes were blocked by DNJ. Therefore, these results show that DNJ delays cellular senescence that is promoted under high glucose condition.

本文言語English
ページ(範囲)63-69
ページ数7
ジャーナルExperimental Gerontology
55
DOI
出版ステータスPublished - 2014 7

ASJC Scopus subject areas

  • 生化学
  • 加齢科学
  • 分子生物学
  • 遺伝学
  • 内分泌学
  • 細胞生物学

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