Zonal differences in DNA synthesis and in transglutaminase activity between perivenous versus periportal regions of regenerating rat liver

Yosuke Ohtake, Akiko Maruko, Soichi Kojima, Tomomi Ono, Tomomi Nagashima, Manabu Fukumoto, Satoko Suyama, Shinya Abe, Naoko Sato, Yasuhito Ohkubo

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We investigated a relationship within zonal differences in DNA synthesis and in transglutaminase (TGase) activity between perivenous versus periportal regions of regenerating rat liver. Using the digitonin/collagenase perfusion technique, hepatocyte subpopulations were isolated from each region at various time points after partial hepatectomy. The amounts of DNA synthesis as well as the levels of TGase mRNA and activity in each subpopulation were measured. Although increased DNA synthesis was observed in both subpopulations with a peak at 24 h after partial hepatectomy, the amount of DNA synthesis in periportal hepatocytes (PPH) was twice as much as that in perivenous hepatocytes (PVH). In PVH, TGase activity peaked at 24 h after partial hepatectomy with a preceding increase in its mRNA expression at 12 h, whereas TGase activity in PPH at 24 h was one-half of that in PVH. As TGase is known to have a growth-arresting activity, our data indicate that relatively higher TGase activity in PVH at 24 h after partial hepatectomy might correlate with relatively lower DNA synthesis in this region compared to periportal region.

Original languageEnglish
Pages (from-to)1758-1762
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number11
DOIs
Publication statusPublished - 2004 Nov

Keywords

  • DNA synthesis
  • Liver regeneration
  • Periportal hepatocyte
  • Perivenous hepatocyte
  • Transglutaminase

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Zonal differences in DNA synthesis and in transglutaminase activity between perivenous versus periportal regions of regenerating rat liver'. Together they form a unique fingerprint.

Cite this