Zebrafish can regenerate endoskeleton in larval pectoral fin but the regenerative ability declines

Keigo Yoshida, Koichi Kawakami, Gembu Abe, Koji Tamura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We show for the first time endoskeletal regeneration in the developing pectoral fin of zebrafish. The developing pectoral fin contains an aggregation plate of differentiated chondrocytes (endochondral disc; primordium for endoskeletal components, proximal radials). The endochondral disc can be regenerated after amputation in the middle of the disc. The regenerated disc sufficiently forms endoskeletal patterns. Early in the process of regenerating the endochondral disc, epithelium with apical ectodermal ridge (AER) marker expression rapidly covers the amputation plane, and mesenchymal cells start to actively proliferate. Taken together with re-expression of a blastema marker gene, msxb, and other developmental genes, it is likely that regeneration of the endochondral disc recaptures fin development as epimorphic limb regeneration does. The ability of endoskeletal regeneration declines during larval growth, and adult zebrafish eventually lose the ability to regenerate endoskeletal components such that amputated endoskeletons become enlarged. Endoskeletal regeneration in the zebrafish pectoral fin will serve as a new model system for successful appendage regeneration in mammals.

Original languageEnglish
Pages (from-to)110-123
Number of pages14
JournalDevelopmental Biology
Issue number2
Publication statusPublished - 2020 Jul 15


  • Endoskeleton
  • Epimorphic
  • Fin
  • Regeneration
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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